Interleukin-4 stimulates collagen gene expression in human fibroblast monolayer cultures. Potential role in fibrosis

FEBS Lett. 1992 May 18;302(3):231-4. doi: 10.1016/0014-5793(92)80448-p.


A role for the cytokines produced by tissue-infiltrated inflammatory cells (mainly T-lymphocytes and mast cells) in the pathophysiology of fibrosis has been suggested by several groups. Among the products of these cells, interleukin-4 (IL-4) might be one of the factors involved in the initiation of the fibrotic process. We studied the effects of recombinant human IL-4 on human fibroblast monolayer cultures. IL-4 (10 and 100 U/ml) induced a dose-dependent increase of collagen production. Non-collagen protein synthesis was not significantly altered. A concomitant increase of pro-alpha 1(I) collagen mRNAs was observed, showing that IL-4 acts at a pre-translational level.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern
  • Cells, Cultured
  • Collagen / biosynthesis
  • Collagen / genetics*
  • Dose-Response Relationship, Drug
  • Fibroblasts / metabolism*
  • Fibrosis
  • Gene Expression*
  • Humans
  • Hydroxylation
  • Interleukin-4 / administration & dosage
  • Interleukin-4 / pharmacology*
  • Proline / metabolism
  • RNA, Messenger / genetics


  • RNA, Messenger
  • Interleukin-4
  • Collagen
  • Proline