Nucleotide sequence of a genomic and a cDNA clone encoding an extracellular alkaline protease of Aspergillus fumigatus

FEMS Microbiol Lett. 1992 Apr 15;71(2):163-8. doi: 10.1016/0378-1097(92)90506-j.


An Aspergillus fumigatus extracellular alkaline protease (ALP) which is an enzyme of the subtilisin family is a potential virulent factor of the fungus. The gene encoding ALP was isolated from a genomic library made from DNA of an A. fumigatus isolate. The nucleotide sequence of this gene was compared to that of a cDNA encoding A. oryzae ALP and to that of a cDNA from A. fumigatus encoding the mature ALP protein. Mature A. fumigatus ALP contains 282 amino acids and is encoded by three exons. The pre-proenzyme has a leader sequence of 121 amino acids.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Aspergillus fumigatus / genetics*
  • Base Sequence
  • Cloning, Molecular
  • DNA Probes
  • Exons / genetics
  • Gene Library
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Sequence Homology, Nucleic Acid
  • Serine Endopeptidases / genetics*
  • Serine Endopeptidases / metabolism


  • DNA Probes
  • Serine Endopeptidases
  • microbial serine proteinases

Associated data

  • GENBANK/Z11580