Trans-scleral diffusion of triamcinolone acetonide

Curr Eye Res. 2005 May;30(5):355-61. doi: 10.1080/02713680590934094.


Purpose: To assess ex vivo human scleral permeability to triamcinolone acetonide (TA).

Methods: The experiments were carried out using scleral samples and a Franz-type vertical diffusion cell. A suspension containing TA was prepared and placed in the donor chamber. The concentration of TA in the receptor chamber was measured by high-performance liquid chromatography (HPLC) assay and expressed as a percentage relative to TA concentration dissolved in the donor chamber. Control experiments using a commercial TA suspension were performed.

Results: TA (+/-SEM) dissolved in the donor suspension was 10.69 +/- 1.28 microg/ml. The diffusion rate of TA varied from 30% after 1 day to 72% after 4 days, after which equilibrium was reached. The human scleral permeability coefficient (P(s) +/- SEM) was 1.47+/- 0.17 x 10(- 5) cm/s.

Conclusions: TA crossed human sclera. The mean amount of drug retained in the sclera increased with time, 4 days being necessary to equilibrate the unidirectional flux. The TA permeability coefficient was comparable to that of other corticosteroids.

MeSH terms

  • Chromatography, High Pressure Liquid
  • Diffusion
  • Glucocorticoids / pharmacokinetics*
  • Humans
  • Middle Aged
  • Permeability
  • Sclera / metabolism*
  • Triamcinolone Acetonide / pharmacokinetics*


  • Glucocorticoids
  • Triamcinolone Acetonide