Proliferation and differentiation of cultured MC3T3-E1 osteoblasts on surface-layer modified hydroxyapatite ceramic with acid and heat treatments

Dent Mater J. 2005 Jun;24(2):207-12. doi: 10.4012/dmj.24.207.

Abstract

Effects of functionally gradient calcium phosphate consisting of hydroxyapatite (HAP) and alpha-tricalcium phosphate (alpha-TCP) on proliferation and differentiation of osteoblasts were evaluated using MC3T3-E1 cells. There were no significant differences in the proliferation of MC3T3-E1 cells among HAP-alpha-TCP functionally gradient calcium phosphate, pure HAP, and cell culture plastic wells. mRNA expressions of type I collagen, alkaline phosphate, and osteocalcine were evaluated as indexes of initial; mid-stage, and late-stage osteoblastic differentiation. Basically, HAP-alpha-TCP functionally gradient calcium phosphate and pure HAP enhanced the expressions of the three markers when compared with that of cell culture plastic wells. For type I collagen and alkaline phosphate expressions, HAP-alpha-TCP functionally gradient calcium phosphate showed the same expression level as pure HAP. For osteocalcine expression, HAP-alpha-TCP functionally gradient calcium phosphate showed a higher level than pure HAP. We concluded, therefore, HAP-alpha-TCP functionally gradient calcium phosphate has good potential to be a bone filler material with high osteoconductivity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Alkaline Phosphatase / biosynthesis
  • Analysis of Variance
  • Animals
  • Bone Substitutes / chemistry
  • Bone Substitutes / pharmacology*
  • Calcium Phosphates / pharmacology*
  • Cell Differentiation / drug effects
  • Cell Proliferation / drug effects
  • Ceramics / chemistry
  • Ceramics / pharmacology*
  • Collagen Type I / biosynthesis
  • Durapatite / chemistry
  • Durapatite / pharmacology*
  • Glycerolphosphate Dehydrogenase / biosynthesis
  • Mice
  • Osteoblasts / drug effects*
  • Osteoblasts / metabolism
  • Osteocalcin / biosynthesis
  • RNA, Messenger / biosynthesis

Substances

  • Bone Substitutes
  • Calcium Phosphates
  • Collagen Type I
  • RNA, Messenger
  • Osteocalcin
  • Durapatite
  • calcium phosphate
  • Glycerolphosphate Dehydrogenase
  • Alkaline Phosphatase