Recent advances in protein splicing: manipulating proteins in vitro and in vivo

Curr Opin Biotechnol. 2005 Aug;16(4):440-6. doi: 10.1016/j.copbio.2005.06.012.


Protein splicing is an intricate self-catalyzed protein rearrangement that converts an inactive protein precursor to biologically active proteins. In the past decade, mechanistic studies and extensive engineering of the naturally occurring protein splicing elements, termed inteins, has led to the development of numerous novel technologies. These intein-based methodologies permit in vitro and in vivo protein processing in ways previously not possible using traditional biochemical and genetic approaches. Inteins have been utilized in the production of protein and peptide arrays, as molecular switches and in the reconstitution of functional proteins by split-gene techniques.

Publication types

  • Review

MeSH terms

  • Animals
  • Drosophila melanogaster / chemistry
  • Drosophila melanogaster / genetics
  • Mycobacterium tuberculosis / chemistry
  • Mycobacterium tuberculosis / genetics
  • Protein Engineering*
  • Protein Splicing / genetics*
  • Trans-Splicing / genetics*