Impaired cytolytic activity in peripheral blood T cells from renal cell carcinoma patients

Clin Immunol. 2005 Oct;117(1):6-11. doi: 10.1016/j.clim.2005.06.010.


Classic T lymphocyte cytotoxicity is mediated through the T cell receptor (TCR). Defects in TCR signal transduction and cytolytic activity have been reported in tumor infiltrating T lymphocytes. We hypothesized that impaired cytotoxicity occurs in peripheral blood T cells from renal cell carcinoma (RCC) that can be reversed by exposure to rhIL-2. Peripheral blood mononuclear cells (PBMC) from 29 RCC patients and 29 healthy volunteers were isolated and cultured in the absence or presence of 10 IU/ml rhIL-2. A redirected cytotoxicity assay that requires TCR signal transduction was used with chromium-labeled P815 target cells, effector PBMC and anti-CD3 antibody. Target cell lysis was measured in "lytic units" (LU). Mean LU from RCC patients was lower than that of healthy volunteers (105.8 LU vs. 194.6 LU, P = 0.025). Exposure to rhIL-2 increased T cell-mediated lysis in both groups. Disruption of T cell cytotoxicity in RCC patients can be overcome by exposure to rhIL-2.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • CD3 Complex / immunology
  • CD3 Complex / metabolism
  • CD8 Antigens / immunology
  • CD8 Antigens / metabolism
  • Carcinoma, Renal Cell / immunology*
  • Cytotoxicity, Immunologic*
  • Female
  • Flow Cytometry
  • Humans
  • Interleukin-2 / immunology
  • Kidney Neoplasms / immunology*
  • Lymphocyte Activation / immunology
  • Male
  • Middle Aged
  • Receptors, Antigen, T-Cell / immunology
  • Recombinant Proteins / immunology
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / metabolism
  • T-Lymphocytes / pathology


  • CD3 Complex
  • CD8 Antigens
  • Interleukin-2
  • Receptors, Antigen, T-Cell
  • Recombinant Proteins