Rapid and sensitive electron-capture gas chromatographic method for the determination of pinazepam and its metabolites in human plasma, urine and milk

J Chromatogr. 1977 May 11;135(1):133-9. doi: 10.1016/s0021-9673(00)86309-8.

Abstract

A rapid, sensitive and specific gas-liquid chromatographic method for the measurement of pinazepam and its metabolites in biological fluids is reported. After a single extraction of the sample with toluene, the organic phase is concentrated and, after chromatography on a 3% OV-17 column, measured with an electron-capture detector. The sensitivity was 1.0 ng/ml for pinazepam and 5.0 ng/ml for its metabolites. Plasma levels and urinary excretion in human volunteers and plasma and milk levels in women suffering from anxiety during breastfeeding are reported.

MeSH terms

  • Anti-Anxiety Agents / analysis*
  • Anti-Anxiety Agents / blood
  • Anti-Anxiety Agents / urine
  • Autoanalysis
  • Benzodiazepines
  • Chromatography, Gas / methods*
  • Female
  • Humans
  • Milk, Human / analysis*
  • Pregnancy

Substances

  • Anti-Anxiety Agents
  • Benzodiazepines
  • pinazepam