Cell death by autoschizis in TRAMP prostate carcinoma cells as a result of treatment by ascorbate: menadione combination

Ultrastruct Pathol. May-Aug 2005;29(3-4):221-35. doi: 10.1080/01913120590951239.

Abstract

A prostate carcinoma cell line derived from the transgenic murine prostate cancer model (TRAMP) was treated with ascorbate (VC) alone, menadione (VK(3)) alone, or a combination of ascorbate:menadione (VC + VK(3)) for 1, 2, and 4 h. Cytotoxic cell alterations examined by light and electron microscopy were treatment-dependent with VC + VK(3) > VC > VK(3). Induced by oxidative stress, these alterations included cytokeletal changes conducive to cytoplasmic blebbing, self-excisions, and progressive nuclear alterations. While the excised parts contained ribosomes, they were devoid of nuclear fragments or other organelles. The organelle-free self-excisions caused an extreme reduction in cell size as well as chromatolysis and karyolysis that were consistent with cell death by autoschizis, but not with apoptosis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology
  • Apoptosis / drug effects*
  • Ascorbic Acid / pharmacology*
  • Cell Line, Tumor
  • Cell Nucleus / drug effects
  • Cell Nucleus / pathology
  • Cell Nucleus / ultrastructure
  • Cell Proliferation / drug effects
  • Cytoplasm / drug effects
  • Cytoplasm / pathology
  • Cytoplasm / ultrastructure
  • Cytoskeleton / drug effects
  • Cytoskeleton / metabolism
  • Cytoskeleton / ultrastructure
  • Drug Synergism
  • Male
  • Mice
  • Mice, Transgenic
  • Microscopy, Electron
  • Prostatic Neoplasms / metabolism
  • Prostatic Neoplasms / pathology
  • Prostatic Neoplasms / ultrastructure
  • Time Factors
  • Vitamin K 3 / pharmacology*

Substances

  • Antioxidants
  • Vitamin K 3
  • Ascorbic Acid