In vitro antileukaemic activity of extracts from berry plant leaves against sensitive and multidrug resistant HL60 cells

Cancer Lett. 2006 May 18;236(2):282-91. doi: 10.1016/j.canlet.2005.05.018. Epub 2005 Jul 20.


The aim of the present study was to determine in vitro antileukaemic activity of extracts obtained from selected berry plant leaves (Fragaria x ananassa Duch. cv Elsanta, raspberry Rubus ideus L. cv Polana and blueberry Vaccinium corymbosum L. cv Bluecrop) against promyelocytic HL60 cell line and its multidrug resistant sublines exhibiting two different MDR phenotypes: HL60/VINC (overexpressing P-glycoprotein) and HL60/DOX (overexpressing MRP1 protein). It was found that the blueberry extract was the most efficient against sensitive HL60 cell line (about 2-fold more active than strawberry and raspberry extracts) but presented much lower activity towards resistant cells. In contrast, strawberry and raspberry extracts exhibited the high cytotoxic activity against sensitive leukaemia HL60 cell line as well as its MDR sublines. The values of resistance factor (RF) found for these extracts were very low lying in the range 0.32/2.0.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / biosynthesis
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
  • Antineoplastic Agents, Phytogenic / chemistry
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Blueberry Plants*
  • Cell Proliferation / drug effects
  • Drug Resistance, Multiple
  • Drug Resistance, Neoplasm
  • Ellagic Acid / analysis
  • Fragaria*
  • Gallic Acid / analysis
  • HL-60 Cells
  • Humans
  • Inhibitory Concentration 50
  • Multidrug Resistance-Associated Proteins / biosynthesis
  • Multidrug Resistance-Associated Proteins / genetics
  • Phenols / analysis
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology
  • Plant Leaves / chemistry
  • Quercetin / analysis


  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Antineoplastic Agents, Phytogenic
  • Multidrug Resistance-Associated Proteins
  • Phenols
  • Plant Extracts
  • Ellagic Acid
  • Gallic Acid
  • Quercetin
  • multidrug resistance-associated protein 1