Cell Type-Specific Involvement of RIG-I in Antiviral Response

Immunity. 2005 Jul;23(1):19-28. doi: 10.1016/j.immuni.2005.04.010.

Abstract

Toll-like receptors (TLRs) play an important role in antiviral response by recognizing viral components. Recently, a RNA helicase, RIG-I, was also suggested to recognize viral double-stranded RNA. However, how these molecules contribute to viral recognition in vivo is poorly understood. We show by gene targeting that RIG-I is essential for induction of type I interferons (IFNs) after infection with RNA viruses in fibroblasts and conventional dendritic cells (DCs). RIG-I induces type I IFNs by activating IRF3 via IkappaB kinase-related kinases. In contrast, plasmacytoid DCs, which produce large amounts of IFN-alpha, use the TLR system rather than RIG-I for viral detection. Taken together, RIG-I and the TLR system exert antiviral responses in a cell type-specific manner.

MeSH terms

  • Animals
  • DEAD Box Protein 58
  • DEAD-box RNA Helicases
  • DNA-Binding Proteins / metabolism
  • Dendritic Cells / immunology*
  • Dendritic Cells / metabolism
  • Dendritic Cells / virology
  • Fibroblasts / immunology*
  • Fibroblasts / metabolism
  • Fibroblasts / virology
  • Gene Targeting
  • I-kappa B Kinase
  • Interferon Regulatory Factor-3
  • Interferon Type I / metabolism
  • Interferon-alpha / metabolism
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / physiology*
  • Mice
  • Newcastle Disease / enzymology
  • Newcastle Disease / immunology
  • Newcastle disease virus / immunology
  • Protein-Serine-Threonine Kinases / metabolism
  • RNA Helicases / genetics
  • RNA Helicases / physiology*
  • RNA Virus Infections / enzymology*
  • RNA Virus Infections / immunology*
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / physiology*
  • Signal Transduction
  • Toll-Like Receptors
  • Transcription Factors / metabolism

Substances

  • DNA-Binding Proteins
  • Interferon Regulatory Factor-3
  • Interferon Type I
  • Interferon-alpha
  • Irf3 protein, mouse
  • Membrane Glycoproteins
  • Receptors, Cell Surface
  • Toll-Like Receptors
  • Transcription Factors
  • Protein-Serine-Threonine Kinases
  • Chuk protein, mouse
  • I-kappa B Kinase
  • Ikbkb protein, mouse
  • Ikbke protein, mouse
  • Ddx58 protein, mouse
  • DEAD Box Protein 58
  • DEAD-box RNA Helicases
  • RNA Helicases