Kin4 kinase delays mitotic exit in response to spindle alignment defects

Mol Cell. 2005 Jul 22;19(2):209-21. doi: 10.1016/j.molcel.2005.05.030.

Abstract

For many polarized cells, it is critical that the mitotic spindle becomes positioned relative to the polarity axis. This is especially important in yeast, where the site of cytokinesis is predetermined. The spindle position checkpoint (SPOC) therefore delays mitotic exit of cells with a mispositioned spindle. One component of the SPOC is the Bub2-Bfa1 complex, an inhibitor of the mitotic exit network (MEN). Here, we show that the Kin4 kinase is a component of the SPOC and as such is essential to delay cell cycle progression of cells with a misaligned spindle. When spindles are correctly oriented, Kin4 and Bub2-Bfa1 are asymmetrically localized to opposite spindle pole bodies (SPBs). Bub2-Bfa1 then becomes inhibited by Cdc5 polo kinase with anaphase onset, a prerequisite for mitotic exit. In response to spindle misalignment, Kin4 and Bub2-Bfa1 are brought together at both SPBs. Kin4 now maintains Bub2-Bfa1 activity by counteracting Cdc5, thereby inhibiting mitotic exit.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Cycle / drug effects
  • Cell Cycle / physiology
  • Cell Cycle Proteins / antagonists & inhibitors
  • Cell Cycle Proteins / chemistry
  • Cell Cycle Proteins / metabolism
  • Cell Cycle Proteins / pharmacology
  • Cytoskeletal Proteins / antagonists & inhibitors
  • Cytoskeletal Proteins / metabolism
  • Cytoskeletal Proteins / pharmacology
  • Microtubules / metabolism
  • Mitosis / drug effects
  • Mitosis / physiology*
  • Protein Kinases / chemistry
  • Protein Kinases / metabolism*
  • Protein Kinases / pharmacology
  • Protein-Serine-Threonine Kinases
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / antagonists & inhibitors
  • Saccharomyces cerevisiae Proteins / chemistry
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Saccharomyces cerevisiae Proteins / pharmacology
  • Spindle Apparatus / drug effects
  • Spindle Apparatus / metabolism*

Substances

  • BFA1 protein, S cerevisiae
  • BUB2 protein, S cerevisiae
  • Cell Cycle Proteins
  • Cytoskeletal Proteins
  • Saccharomyces cerevisiae Proteins
  • Protein Kinases
  • KIN4 kinase, S cerevisiae
  • Protein-Serine-Threonine Kinases
  • CDC5 protein, S cerevisiae