Inhibition by adiponectin of IL-8 production by human macrophages upon coculturing with late apoptotic cells

Biochem Biophys Res Commun. 2005 Sep 9;334(4):1180-3. doi: 10.1016/j.bbrc.2005.07.016.

Abstract

Adiponectin, an adipocyte-derived hormone, reportedly suppresses the production of TNF-alpha and IL-6 by LPS-stimulated human or porcine macrophages, and the phagocytosis of microbeads by human macrophages. In this study, we used a high molecular weight form of adiponectin purified from human plasma to examine its effects on the phagocytosis of late apoptotic cells by human macrophages and the subsequent IL-8 production. Adiponectin suppressed both the phagocytosis of apoptotic cells and the IL-8 production. In contrast, adiponectin augmented both the phagocytosis of apoptotic cells and the IL-8 production in the presence of LPS. These results suggest that adiponectin is not an anti-inflammatory hormone but rather a dual modulator of innate responses.

MeSH terms

  • Adiponectin
  • Apoptosis / drug effects
  • Apoptosis / physiology*
  • Cells, Cultured
  • Coculture Techniques / methods
  • Dose-Response Relationship, Drug
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / physiology*
  • Humans
  • Intercellular Signaling Peptides and Proteins / metabolism*
  • Interleukin-8 / biosynthesis*
  • Macrophages / cytology
  • Macrophages / drug effects
  • Macrophages / metabolism*
  • Phagocytosis / drug effects
  • Phagocytosis / physiology*
  • T-Lymphocytes / cytology
  • T-Lymphocytes / physiology*

Substances

  • Adiponectin
  • Intercellular Signaling Peptides and Proteins
  • Interleukin-8