Ganodermin, an antifungal protein from fruiting bodies of the medicinal mushroom Ganoderma lucidum

Hexiang Wang; T B Ng

doi:10.1016/j.peptides.2005.06.009

Ganodermin, an antifungal protein from fruiting bodies of the medicinal mushroom Ganoderma lucidum

Peptides. 2006 Jan;27(1):27-30. doi: 10.1016/j.peptides.2005.06.009. Epub 2005 Jul 21.

Authors

Hexiang Wang¹, T B Ng

Affiliation

¹ Department of Microbiology, College of Biological Science, China Agricultural University, Beijing, China.

PMID: 16039755
DOI: 10.1016/j.peptides.2005.06.009

Abstract

A 15-kDa antifungal protein, designated ganodermin, was isolated from the medical mushroom Ganoderma lucidum. The isolation procedure utilized chromatography on DEAE-cellulose, Affi-gel blue gel, CM-Sepharose and Superdex 75. Ganodermin was unadsorbed on DEAE-cellulose and adsorbed on Affi-gel blue gel and CM-Sepharose. Ganodermin inhibited the mycelial growth of Botrytis cinerea, Fusarium oxysporum and Physalospora piricola with an IC50 value of 15.2 microM, 12.4 microM and 18.1 microM, respectively. It was devoid of hemagglutinating, deoxyribonuclease, ribonuclease and protease inhibitory activities.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Antifungal Agents / chemistry*
Antifungal Agents / pharmacology*
Botrytis / drug effects
Chromatography, Ion Exchange
Dose-Response Relationship, Drug
Fruiting Bodies, Fungal / chemistry*
Fungal Proteins / isolation & purification*
Fungal Proteins / pharmacology
Molecular Sequence Data
Reishi / chemistry*

Substances

Antifungal Agents
Fungal Proteins