In vitro micropropagation of Boswellia ovalifoliolata

Z Naturforsch C J Biosci. May-Jun 2005;60(5-6):505-7. doi: 10.1515/znc-2005-5-621.

Abstract

A protocol for micropropagation of Boswellia ovalifoliolata Bal & Henry (Burseraceae) was developed using cotyledonary nodal explant on Murashige and Skoog modified medium (MS). A comparative study of micropropagation with 6-benzyladenine, kinetin and thidiazuron along with 1-naphthalene acetic acid (0.054 microM) was conducted. The highest shoot multiplication (7.1 +/- 0.2 shoots per node) was achieved in 50 d on MS supplemented with thidiazuron (2.72 microM). Excised shoot cuttings of 3.0 cm were placed on the MS basal medium supplemented with indole-3-acetic acid and indole-3-butyric acid alone and in combinations for rooting. Activated charcoal (100 mg l(-1)) and polyvinylpyrrolidone (40 mg l(-1)) were added to the medium to prevent browning of cultures. The regenerated plantlets have been successfully acclimatized and transferred to soil.

MeSH terms

  • Adenine / analogs & derivatives
  • Adenine / pharmacology
  • Aminobutyrates / pharmacology
  • Boswellia / physiology*
  • Cell Culture Techniques
  • Cell Division / drug effects
  • Germination / physiology*
  • Kinetin
  • Phenylurea Compounds / pharmacology
  • Plant Growth Regulators / pharmacology
  • Plant Shoots / cytology
  • Plant Shoots / drug effects
  • Plant Shoots / physiology
  • Reproduction
  • Seeds / physiology*
  • Thiadiazoles / pharmacology

Substances

  • Aminobutyrates
  • Phenylurea Compounds
  • Plant Growth Regulators
  • Thiadiazoles
  • thidiazuron
  • 2-amino-4-phenylbutyric acid
  • Adenine
  • Kinetin