A monoclonal antibody recognizing Golgi apparatus produced using affinity purified material from a patient with connective tissue disease

Scand J Rheumatol. 1992;21(3):109-15. doi: 10.3109/03009749209095080.


Serum antibodies recognizing the Golgi apparatus have been reported in patients with connective tissue diseases, but little is known of their significance. Serum from a systemic lupus erythematosus patient with polymyositis was found to have high titers of anti-Golgi apparatus antibody. This serum recognized a 64 kD polypeptide in immunoblotting with HEp-2 cells. To verify that the 64 kD polypeptide was associated with the Golgi apparatus and to characterize which Golgi component was recognized, a monoclonal antibody was produced. IgG, isolated from this serum, was used in affinity chromatography to produce purified material which was used to generate a mouse monoclonal antibody. The monoclonal antibody had an indirect immunofluorescent pattern identical to that produced by the patient's serum, and similarly recognized a 64kD polypeptide in immunoblotting. A 59 kD polypeptide was also recognized by the monoclonal antibody, suggesting that the antigens recognized by the monoclonal and serum antibodies may be only partially identical. The antigen appears to be a glycoprotein and an integral component of the Golgi cisternae membranes.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Aged
  • Antibodies, Monoclonal / analysis
  • Antibodies, Monoclonal / immunology*
  • Antibody Affinity / immunology
  • Cells, Cultured
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Golgi Apparatus / immunology*
  • Golgi Apparatus / ultrastructure
  • Humans
  • Immunoblotting
  • Immunoenzyme Techniques
  • Immunoglobulin G / immunology
  • Lupus Erythematosus, Systemic / immunology*
  • Lupus Erythematosus, Systemic / pathology
  • Microscopy, Immunoelectron
  • Tumor Cells, Cultured / chemistry
  • Tumor Cells, Cultured / immunology
  • Tumor Cells, Cultured / ultrastructure
  • Wheat Germ Agglutinins / immunology


  • Antibodies, Monoclonal
  • Immunoglobulin G
  • Wheat Germ Agglutinins