Abstract
The use of specific quenched fluorescent substrates (QFS) provides a rapid and sensitive method to measure peptidase activity, and is readily adaptable to high-throughput screening of potential peptidase inhibitors. In this chapter, we discuss general considerations for the development of QFS assays, and describe in detail an assay protocol for the mammalian metallopeptidase, endothelin-converting enzyme.
MeSH terms
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Amino Acid Sequence
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Animals
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Aspartic Acid Endopeptidases / analysis
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Aspartic Acid Endopeptidases / metabolism
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Bradykinin / chemistry
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Endothelin-1 / chemistry
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Endothelin-Converting Enzymes
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Fluorescent Dyes* / chemistry
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Metalloendopeptidases / analysis
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Metalloendopeptidases / metabolism
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Molecular Biology / methods
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Molecular Sequence Data
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Peptide Hydrolases / analysis*
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Peptide Hydrolases / metabolism
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Peptides / chemistry
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Substrate Specificity
Substances
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Endothelin-1
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Fluorescent Dyes
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Peptides
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Peptide Hydrolases
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Aspartic Acid Endopeptidases
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Metalloendopeptidases
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Endothelin-Converting Enzymes
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Bradykinin