Herpes Simplex Virus Immediate Early Gene Expression in the Absence of Transinduction by Vmw65 Varies During the Cell Cycle

Virology. 1992 Jul;189(1):196-202. doi: 10.1016/0042-6822(92)90695-l.


The requirement for the herpes simplex virus type 1 (HSV-1) protein Vmw65 (VP16) for activation of immediate early (IE) gene expression was examined in synchronized HeLa cells. Analyses of IE RNA levels were conducted during infection with a viral Vmw65 mutant, in1814. The results revealed an increased requirement for Vmw65 when cultures reached G2 phase of the cell cycle. The levels of IE RNAs 1, 2, and 4 were reduced 5-10 times more in G2 than G1/S for in1814-infected cells when compared to cells infected with wild-type virus or 1814R (a rescued virus), and similar but smaller effects were observed on IE RNA 3 levels. The relative decrease at G2 was reversed by resynchronization of cells to G1/S. Mutant in1814 formed plaques less efficiently on cells at G2 than on cells synchronized at G1/S. The results show that, in the absence of functional Vmw65, HSV-1 IE gene expression and replication vary during the cell cycle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cell Cycle*
  • G2 Phase
  • HeLa Cells
  • Herpes Simplex / metabolism*
  • Humans
  • Immediate-Early Proteins*
  • Molecular Sequence Data
  • RNA, Viral / analysis
  • Transcription, Genetic*
  • Transcriptional Activation*
  • Viral Proteins / metabolism*


  • IE1 protein, Human herpesvirus 1
  • Immediate-Early Proteins
  • RNA, Viral
  • Viral Proteins