A new RNA synthetic method with a 2'-O-(2-cyanoethoxymethyl) protecting group

Tadaaki Ohgi; Yutaka Masutomi; Kouichi Ishiyama; Hidetoshi Kitagawa; Yoshinobu Shiba; Junichi Yano

doi:10.1021/ol051151f

A new RNA synthetic method with a 2'-O-(2-cyanoethoxymethyl) protecting group

Org Lett. 2005 Aug 4;7(16):3477-80. doi: 10.1021/ol051151f.

Authors

Tadaaki Ohgi¹, Yutaka Masutomi, Kouichi Ishiyama, Hidetoshi Kitagawa, Yoshinobu Shiba, Junichi Yano

Affiliation

¹ Discovery Research Laboratories, Nippon Shinyaku Co. Ltd., 3-14-1 Sakura, Tsukuba City, Ibaraki 305-0003, Japan. t.ohgi@po.nippon-shinyaku.co.jp

PMID: 16048321
DOI: 10.1021/ol051151f

Abstract

A novel method for the synthesis of RNA oligomers with 2-cyanoethoxymethyl (CEM) as the 2'-hydroxyl protecting group has been developed. The new method allows the synthesis of oligoribonucleotides with an efficiency and final purity comparable to that obtained in DNA synthesis. [structure: see text]

MeSH terms

Base Sequence
Models, Chemical
Molecular Sequence Data
Molecular Structure
Nitriles / chemistry*
RNA / chemical synthesis*
RNA / chemistry

Substances

Nitriles
RNA