Murine malaria parasite sequestration: CD36 is the major receptor, but cerebral pathology is unlinked to sequestration

Proc Natl Acad Sci U S A. 2005 Aug 9;102(32):11468-73. doi: 10.1073/pnas.0503386102. Epub 2005 Jul 28.


Sequestration of malaria-parasite-infected erythrocytes in the microvasculature of organs is thought to be a significant cause of pathology. Cerebral malaria (CM) is a major complication of Plasmodium falciparum infections, and PfEMP1-mediated sequestration of infected red blood cells has been considered to be the major feature leading to CM-related pathology. We report a system for the real-time in vivo imaging of sequestration using transgenic luciferase-expressing parasites of the rodent malaria parasite Plasmodium berghei. These studies revealed that: (i) as expected, lung tissue is a major site, but, unexpectedly, adipose tissue contributes significantly to sequestration, and (ii) the class II scavenger-receptor CD36 to which PfEMP1 can bind is also the major receptor for P. berghei sequestration, indicating a role for alternative parasite ligands, because orthologues of PfEMP1 are absent from rodent malaria parasites, and, importantly, (iii) cerebral complications still develop in the absence of CD36-mediated sequestration, dissociating parasite sequestration from CM-associated pathology. Real-time in vivo imaging of parasitic processes may be used to evaluate the molecular basis of pathology and develop strategies to prevent pathology.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / parasitology
  • Adipose Tissue / pathology
  • Animals
  • Brain / parasitology
  • Brain / pathology
  • CD36 Antigens / metabolism*
  • Erythrocytes / parasitology*
  • Gene Transfer Techniques
  • Green Fluorescent Proteins
  • Luciferases / metabolism
  • Lung / parasitology
  • Lung / pathology
  • Malaria, Cerebral / pathology*
  • Malaria, Cerebral / physiopathology*
  • Mice
  • Microscopy, Fluorescence / methods
  • Plasmodium berghei / genetics*
  • Plasmodium berghei / metabolism
  • Protozoan Proteins / metabolism
  • Time Factors


  • CD36 Antigens
  • Protozoan Proteins
  • erythrocyte membrane protein 1, Plasmodium falciparum
  • Green Fluorescent Proteins
  • Luciferases