Pesticide resistance via transposition-mediated adaptive gene truncation in Drosophila

Science. 2005 Jul 29;309(5735):764-7. doi: 10.1126/science.1112699.

Abstract

To study adaptation, it is essential to identify multiple adaptive mutations and to characterize their molecular, phenotypic, selective, and ecological consequences. Here we describe a genomic screen for adaptive insertions of transposable elements in Drosophila. Using a pilot application of this screen, we have identified an adaptive transposable element insertion, which truncates a gene and apparently generates a functional protein in the process. The insertion of this transposable element confers increased resistance to an organophosphate pesticide and has spread in D. melanogaster recently.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adaptation, Physiological
  • Alleles
  • Amino Acid Substitution
  • Animals
  • Azinphosmethyl / pharmacology
  • Base Sequence
  • Choline / metabolism
  • Crosses, Genetic
  • DNA Transposable Elements*
  • Drosophila / drug effects
  • Drosophila / genetics
  • Drosophila / physiology
  • Drosophila Proteins / chemistry
  • Drosophila Proteins / genetics*
  • Drosophila Proteins / physiology
  • Drosophila melanogaster / drug effects
  • Drosophila melanogaster / genetics*
  • Drosophila melanogaster / physiology
  • Evolution, Molecular*
  • Exons
  • Female
  • Gene Expression
  • Genes, Insect*
  • Haplotypes
  • Insecticide Resistance / genetics*
  • Insecticides / pharmacology
  • Introns
  • Long Interspersed Nucleotide Elements
  • Molecular Sequence Data
  • Mutation
  • Polymorphism, Genetic
  • Recombination, Genetic
  • Selection, Genetic

Substances

  • DNA Transposable Elements
  • Drosophila Proteins
  • Insecticides
  • Azinphosmethyl
  • Choline