Antibody to eosinophil cationic protein suppresses dextran sulfate sodium-induced colitis in rats

World J Gastroenterol. 2005 Aug 7;11(29):4505-10. doi: 10.3748/wjg.v11.i29.4505.


Aim: To produce an antibody against rat eosinophil cationic protein (ECP) and to examine the effects of the antibody in rats with dextran sulfate sodium (DSS)-induced colitis.

Methods: An antibody was raised against rat ECP. Rats were treated with 3% DSS in drinking water for 7 d and received the antibody or normal serum. The colons were examined histologically and correlated with clinical symptoms. Immunohistochemistry and Western blot analysis were estimated as a grade of inflammation.

Results: The ECP antibody stained the activated eosinophils around the injured crypts in the colonic mucosa. Antibody treatment reduced the severity of colonic ulceration and acute clinical symptoms (diarrhea and/or blood-stained stool). Body weight gain was significantly greater and the colon length was significantly longer in anti-ECP-treated rats than in normal serum-treated rats. Expression of ECP in activated eosinophils was associated with the presence of erosions and inflammation. The number of Ki-67-positive cells in the regenerated surface epithelium increased in anti-ECP-treated rats compared with normal serum-treated rats. Western blot analysis revealed reduced expression of macrophage migration inhibitory factor (MIF) in anti-ECP-treated rats.

Conclusion: Our results indicate that treatment with ECP antibody, improved DSS-induced colitis in rats, possibly by increasing the regenerative activity of the colonic epithelium and downregulation of the immune response, and suggest that anti-ECP may promote intestinal wound healing in patients with ulcerative colitis (UC).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies / pharmacology*
  • Anticoagulants
  • Colitis, Ulcerative / chemically induced
  • Colitis, Ulcerative / prevention & control*
  • Colitis, Ulcerative / therapy*
  • Dextran Sulfate
  • Eosinophil Cationic Protein / chemistry
  • Eosinophil Cationic Protein / immunology*
  • Immunotherapy / methods*
  • Male
  • Molecular Sequence Data
  • Rats
  • Rats, Wistar


  • Antibodies
  • Anticoagulants
  • Dextran Sulfate
  • Eosinophil Cationic Protein