Novel DNA vaccine based on hepatitis B virus core gene induces specific immune responses in Balb/c mice

World J Gastroenterol. 2005 Aug 7;11(29):4583-6. doi: 10.3748/wjg.v11.i29.4583.


Aim: To investigate the immunogenicity of a novel DNA vaccine, pSW3891/HBc, based on HBV core gene in Balb/c mice.

Methods: A novel DNA vaccine, pSW3891/HBc, encoding HBV core gene was constructed using a vector plasmid pSW3891. Balb/c mice were immunized with either pSW3891/HBc or empty vector DNA via gene gun. IgG anti-HBc responses in mouse sera were demonstrated by ELISA. Specific cytotoxicity of cytotoxic T lymphocytes (CTLs) of mice was quantitatively measured by lactate dehydrogenase release assay.

Results: HBcAg was expressed effectively in 293T cell line transiently transfected with pSW3891/HBc. Strong IgG anti-HBc responses were elicited in mice immunized with pSW3891/HBc. The end-point titers of anti-HBc reached the highest 1:97,200, 4 wk after the third immunization. The specific CTL killing with the highest specific lysis reached 73.25% at effector:target ratio of 20:1 in mice that received pSW3891/HBc DNA vaccine.

Conclusion: pSW3891/HBc vaccination elicits specific anti-HBc response and induces HBc-specific CTL response in immunized Balb/c mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Female
  • Hepatitis B Core Antigens / genetics*
  • Hepatitis B Core Antigens / immunology
  • Hepatitis B Vaccines / genetics*
  • Hepatitis B Vaccines / immunology
  • Hepatitis B Vaccines / pharmacology
  • Hepatitis B, Chronic / immunology
  • Hepatitis B, Chronic / therapy*
  • Mice
  • Mice, Inbred BALB C
  • Plasmids
  • T-Lymphocytes, Cytotoxic / immunology
  • Vaccines, DNA / genetics*
  • Vaccines, DNA / immunology
  • Vaccines, DNA / pharmacology


  • Hepatitis B Core Antigens
  • Hepatitis B Vaccines
  • Vaccines, DNA