Molecular analysis of the yellow locus of Drosophila

EMBO J. 1986 Dec 20;5(13):3597-605.

Abstract

The yellow (y) locus of Drosophila controls the pattern of pigmentation of the adult cuticle and larval mouth parts. Mutations of y belong to two phenotypic classes; type1 mutants exhibit a total loss of pigmentation from all parts of the cuticle and type 2 mutants show allele-specific mosaic pigmentation patterns. We have cloned y by P-element tagging followed by chromosome walking. We demonstrate that a 2.1-kb transcript which spans 4.6 kb of genomic sequence is the y transcript. The location of this transcript and the putative protein it encodes has been defined by nuclease protection, primer extension and DNA sequencing experiments. A 12-kb genomic fragment containing this transcript along with 2.8 kb of 5'-flanking sequence and 4.6 kb of 3'-flanking sequence is sufficient for the wild-type expression of the larval and adult cuticle phenotypes in germ-line transformants. The majority (12/14) of type1 mutants show structural lesions within exon coding portions of y. Most (6/7) of the type2 structural lesions map 5' to the start of y transcription. One type2 mutant which contains an insertion within the 5'-transcribed but non-translated region appears to have resulted from the internal deletion of a P-element associated with a type1 allele.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Drosophila / genetics*
  • Drosophila / growth & development
  • Drosophila Proteins / genetics*
  • Gene Expression Regulation, Developmental
  • Larva / genetics
  • Molecular Sequence Data
  • Mutation

Substances

  • Drosophila Proteins
  • y protein, Drosophila