Krox20 stimulates adipogenesis via C/EBPbeta-dependent and -independent mechanisms

Cell Metab. 2005 Feb;1(2):93-106. doi: 10.1016/j.cmet.2004.12.009.


Krox20 is a zinc finger-containing transcription factor that is abundantly expressed in adipose tissue. However, its role in fat cell differentiation has not been established. In cultured 3T3-L1 cells, Krox20 is rapidly induced by serum stimulation. Overexpression of Krox20 in both 3T3-L1 preadipocytes and multipotent NIH3T3 cells promotes adipogenesis in a hormone-dependent manner. Conversely, RNAi-mediated loss of Krox20 function reduced adipogenesis in 3T3-L1 cells. Ectopic expression of Krox20 can transactivate the C/EBPbeta promoter and increase C/EBPbeta gene expression in 3T3-L1 preadipocytes. RNAi-mediated knockdown of C/EPBbeta diminished Krox20's proadipogenic effect. Finally, coexpression of Krox20 and C/EBPbeta in naive NIH3T3 cells resulted in the pronounced induction of a fully differentiated adipocyte phenotype, an effect previously observed only with PPARgamma. These data indicate that Krox20 is necessary for adipogenesis and that, when overexpressed, Krox20 potently stimulates adipogenesis via C/EBPbeta-dependent and -independent mechanisms.

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / cytology
  • Adipocytes / metabolism
  • Animals
  • Blotting, Western
  • CCAAT-Enhancer-Binding Protein-beta / metabolism*
  • Cell Differentiation
  • Cell Line
  • Cell Lineage
  • DNA-Binding Proteins / metabolism
  • DNA-Binding Proteins / physiology*
  • Early Growth Response Protein 2
  • Gene Silencing
  • Genes, Reporter
  • Hormones / metabolism
  • Luciferases / metabolism
  • Mice
  • Molecular Sequence Data
  • NIH 3T3 Cells
  • PPAR gamma / metabolism
  • Phenotype
  • Plasmids / metabolism
  • Promoter Regions, Genetic
  • RNA Interference
  • RNA, Small Interfering / metabolism
  • Retroviridae / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Time Factors
  • Transcription Factors / metabolism
  • Transcription Factors / physiology*
  • Transcriptional Activation
  • Transfection
  • Zinc Fingers


  • CCAAT-Enhancer-Binding Protein-beta
  • DNA-Binding Proteins
  • Early Growth Response Protein 2
  • Egr2 protein, mouse
  • Hormones
  • PPAR gamma
  • RNA, Small Interfering
  • Transcription Factors
  • Luciferases

Associated data

  • GENBANK/X06746
  • GENBANK/X62600