Therapeutic vaccination with papillomavirus E6 and E7 long peptides results in the control of both established virus-induced lesions and latently infected sites in a pre-clinical cottontail rabbit papillomavirus model

Vaccine. 2005 Nov 1;23(45):5271-80. doi: 10.1016/j.vaccine.2005.04.049. Epub 2005 Jul 11.


This study was performed to test the therapeutic efficacy of overlapping long E6 and E7 peptides, containing both CD4+ T-helper and CD8+ CTL epitopes, on CRPV-induced lesions, which is an appropriate pre-clinical model for HPV diseases, including recurrent respiratory papillomatosis (RRP). Therapeutic peptide vaccination was able to significantly control wart growth (p < 0.01) and abrogate latent CRPV infection (p = 0.0006) compared to controls. Vaccination was associated with a T(H)1 T cell response, as suggested by a strong DTH skin test, antigen-specific proliferation of PBMC and a minimal IgG antibody response. Thus, this study shows promise for treatment of RRP by vaccination with long peptides.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cell Proliferation
  • Cottontail rabbit papillomavirus / immunology*
  • DNA, Viral / analysis
  • Epithelial Cells / immunology
  • Genes, MHC Class I / immunology
  • Genes, MHC Class II / genetics
  • Hypersensitivity, Delayed
  • Immunity, Cellular / immunology
  • Immunohistochemistry
  • Monocytes / immunology
  • Oncogene Proteins, Viral / immunology*
  • Rabbits
  • Skin Tests
  • T-Lymphocytes, Helper-Inducer / immunology
  • Tumor Virus Infections / immunology
  • Tumor Virus Infections / therapy*
  • Tumor Virus Infections / virology
  • Vaccination
  • Virus Latency
  • Warts / immunology
  • Warts / pathology
  • Warts / prevention & control


  • DNA, Viral
  • E6 protein, Cottontail rabbit papillomavirus
  • E7 protein, cottontail rabbit papillomavirus
  • Oncogene Proteins, Viral