Product and product-independent induction of butane oxidation in Pseudomonas butanovora

FEMS Microbiol Lett. 2005 Sep 1;250(1):111-6. doi: 10.1016/j.femsle.2005.06.058.

Abstract

Pseudomonas butanovora grows on butane by means of an inducible soluble alkane monooxygenase (sBMO). The induction of sBMO was studied using the wild type and a sBMO reporter strain. The reporter strain has the lacZ::kan cassette inserted into bmoX, the gene that encodes the alpha-subunit of the hydroxylase of sBMO. The beta-galactosidase activity in the reporter strain was not induced by butane, but was induced by 1-butanol and butyraldehyde. P. butanovora expressed sBMO product-independent activity at 3.0+/-1 nmol ethylene oxide min(-1) mg protein(-1) in stationary phase. The sBMO product-independent activity likely primes the expression of sBMO by butane.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 1-Butanol / metabolism
  • 1-Butanol / pharmacology
  • Aldehydes / metabolism
  • Aldehydes / pharmacology
  • Butanes / metabolism*
  • Butanes / pharmacology
  • Cytochrome P-450 CYP4A / genetics
  • Cytochrome P-450 CYP4A / metabolism
  • DNA, Bacterial / genetics
  • Genes, Reporter
  • Lac Operon
  • Models, Biological
  • Mutagenesis, Insertional
  • Oxidation-Reduction
  • Pseudomonas / drug effects
  • Pseudomonas / genetics
  • Pseudomonas / metabolism*
  • RNA, Bacterial / genetics
  • RNA, Bacterial / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Solubility

Substances

  • Aldehydes
  • Butanes
  • DNA, Bacterial
  • RNA, Bacterial
  • RNA, Messenger
  • butane
  • 1-Butanol
  • Cytochrome P-450 CYP4A
  • butyraldehyde