Molecular cloning of human epsilon-globin gene

Proc Natl Acad Sci U S A. 1979 Nov;76(11):5435-9. doi: 10.1073/pnas.76.11.5435.


Human beta-like globin genes were investigated by use of rabbit beta-globin cDNA plasmid as a cross-species hybridization probe. Normal and beta 0/delta beta 0 thalassemic DNA were compared by filter hybridization procedures. It proved possible to demonstrate that the rabbit probe detected G gamma, A gamma, delta, beta, beta 0, and delta beta 0 human globin genes as well as an additional unidentified beta-like globin gene. By use of an agarose gel elution procedure, fractions of HindIII-digested DNA enriched for beta-like globin genes were purified. One of these fractions, 8.0 kilobases in size, was clonedinto lambda 788, and EK2 lambda HindIII vector. A positive clone was obtained and characterized by restriction mapping and sequence analysis. The sequence data obtained predicted an amino acid sequence that exactly matches a part of human epsilon-globin. The human non-alpha-globin locus is now nearly complete. delta, beta, and gamma human globin genes have already been cloned and analyzed. We describe here the cloning of the remaining non-alpha-globin gene, epsilon.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Bacteriophage lambda / genetics
  • Base Sequence
  • DNA / genetics
  • DNA Restriction Enzymes
  • DNA, Recombinant
  • Globins / genetics*
  • Humans
  • Rabbits


  • DNA, Recombinant
  • Globins
  • DNA
  • DNA Restriction Enzymes

Associated data

  • GENBANK/J00093
  • GENBANK/J00094
  • GENBANK/J00096
  • GENBANK/J00158
  • GENBANK/J00159
  • GENBANK/J00160
  • GENBANK/J00161
  • GENBANK/J00162
  • GENBANK/J00163
  • GENBANK/J00164
  • GENBANK/J00165
  • GENBANK/J00166
  • GENBANK/J00167
  • GENBANK/J00168
  • GENBANK/J00169
  • GENBANK/J00170
  • GENBANK/J00171
  • GENBANK/J00172
  • GENBANK/J00173
  • GENBANK/J00174
  • GENBANK/J00175
  • GENBANK/J00177
  • GENBANK/J00178
  • GENBANK/J00179
  • GENBANK/K01239
  • GENBANK/K01890
  • GENBANK/K02544
  • GENBANK/M18047
  • GENBANK/M19067
  • GENBANK/X00423