cDNA clone to chick corneal chondroitin/dermatan sulfate proteoglycan reveals identity to decorin

Arch Biochem Biophys. 1992 Jul;296(1):190-7. doi: 10.1016/0003-9861(92)90562-b.


A 1.6-kb cDNA clone was isolated by screening a library prepared from chick corneal mRNA with a cDNA clone to bovine decorin. The cDNA contained an open reading frame coding for a M(r) 39,683 protein. A 19-amino-acid match with sequence from the N-terminus of core protein from the corneal chondroitin/dermatan sulfate proteoglycan confirmed the clone as a corneal proteoglycan and the homology with human and bovine decorin confirmed its identity as decorin. Structural features of the deduced sequence include a 16-amino-acid signal peptide, a 14-amino-acid propeptide, cysteine residues at the N- and C-terminal regions, and a central leucine-rich region (comprising 63% of the protein) containing nine repeats of the sequence LXXLXLXXNXL/I. Chick decorin contains three variations of this sequence that are tandemly linked to form a unit and three units tandemly linked to form the leucine-rich region. The presence of beta bend amino acids flanking the units may serve to delineate the units as structural elements of the leucine-rich region. Sequence homology within the repeats and the spacing of the repeats suggest that this region arose by duplication. Chick decorin primarily differs from mammalian decorins in the 19-amino-acid sequence that starts the N-terminus of the core protein. Within this region, the serine that serves as a potential acceptor for the chondroitin/dermatan sulfate side chain is preceded by a glycine instead of being followed by a glycine as it is in the mammalian decorins and all other mammalian proteoglycans.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cattle
  • Chickens
  • Chondroitin Sulfate Proteoglycans / genetics*
  • Cloning, Molecular
  • Cornea / physiology*
  • DNA / genetics*
  • DNA / isolation & purification
  • Decorin
  • Dermatan Sulfate / genetics*
  • Extracellular Matrix Proteins
  • Gene Library
  • Glycosylation
  • Humans
  • Molecular Sequence Data
  • Proteoglycans / genetics*
  • RNA, Messenger / genetics
  • RNA, Messenger / isolation & purification
  • Repetitive Sequences, Nucleic Acid
  • Sequence Homology, Nucleic Acid


  • Chondroitin Sulfate Proteoglycans
  • DCN protein, human
  • Decorin
  • Extracellular Matrix Proteins
  • Proteoglycans
  • RNA, Messenger
  • dermatan sulfate proteoglycan
  • Dermatan Sulfate
  • DNA

Associated data

  • GENBANK/L05509
  • GENBANK/L05510
  • GENBANK/L05930
  • GENBANK/S76223
  • GENBANK/S76225
  • GENBANK/S76228
  • GENBANK/S76232
  • GENBANK/S76235
  • GENBANK/X63431
  • GENBANK/X63797