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, 40 (8), 1000-4

Measurement of Serum Salicylate Levels by Solid-Phase Extraction and Desorption/Ionization on Silicon Mass Spectrometry

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Measurement of Serum Salicylate Levels by Solid-Phase Extraction and Desorption/Ionization on Silicon Mass Spectrometry

Shoji Okuno et al. J Mass Spectrom.

Abstract

The applicability of the matrix-free laser desorption/ionization on silicon mass spectrometry (DIOS-MS) to measuring serum drug levels was examined by analyzing serum salicylic acid. The optimized and simple solid-phase extraction (SPE) allowed good recovery, 88.9 +/- 5.8%, for 1.4 mM (200 mg/L) of salicylic acid in serum. The negative ion MS allowed measurements of deprotonated molecules without interference from other signals. Using a deuterium-labeled internal standard, good linearity was obtained in the 0.14 to 4.2 mM (20-600 mg/L) range, which was sufficient for monitoring the therapeutic anti-inflammatory dose. SPE followed by DIOS-MS is anticipated to be a method of measuring drug levels in blood and may allow high throughput analysis.

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