A naturally occurring peptide recognized by alloreactive CD8+ cytotoxic T lymphocytes in association with a class I MHC protein

Cell. 1992 Jun 12;69(6):989-98. doi: 10.1016/0092-8674(92)90617-l.


The antigenic structures that initiate T cell responses to foreign (allogeneic) cells have long attracted considerable interest. We have purified and sequenced a peptide from mouse spleen that is recognized in association with the class I MHC protein H-2Ld by 2C, an alloreactive CD8+ T cell clone. The peptide (LSP-FPFDL) greatly enhances the susceptibility of Ld+ cells to lysis by 2C, and this activity is completely blocked by a clonotypic antibody against the 2C T cell receptor. Thus, this study characterizes the naturally occurring peptide moiety of an MHC-I/peptide complex recognized by alloreactive CD8+ T cells. The peptide, which occurs in the thymus of MHC-disparate mice, can be used to study T cell development in mice expressing transgenes for the 2C T cell receptor.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • CD8 Antigens / analysis
  • Chromatography, High Pressure Liquid
  • Clone Cells
  • Cytotoxicity, Immunologic
  • H-2 Antigens / metabolism*
  • Immunity, Cellular
  • In Vitro Techniques
  • Mice
  • Molecular Sequence Data
  • Peptides / immunology*
  • Peptides / isolation & purification
  • Peptides / metabolism
  • Pronase / pharmacology
  • Protein Binding
  • Receptors, Antigen, T-Cell / metabolism*
  • T-Lymphocytes, Cytotoxic / immunology*


  • CD8 Antigens
  • H-2 Antigens
  • Peptides
  • Receptors, Antigen, T-Cell
  • Pronase