Objective: To observe the different mRNA levels of Candida albicans ALS gene family between planktonic and biofilm-grown cells.
Methods: ATCC 90038 and a wild strain of Candida albicans, biofilm models in vitro were formed on glass slides. After 48 hours' incubation, the biofilm-grown cells were harvested. Half-quantification of ALS1 and ALS4 mRNA was based on the amplification by one-step RT-PCR.
Results: The amounts of ALS1 and ALS4 mRNA of the wild strain in biofilm increased comparing with planktonic cells, while ATCC 90038 didn't.
Conclusion: The members of ALS gene family may play important roles in the course of Candida albicans biofilm formation.