Dietary Trans Fatty Acids Alter Diaphragm Phospholipid Fatty Acid Composition, Triacylglycerol Content and Glucose Transport in Rats

Br J Nutr. 2005 Jun;93(6):829-33. doi: 10.1079/BJN20051442.


The present study evaluates the effect of dietary trans fatty acids on diaphragm phospholipid fatty acid composition, intramyocellular triacylglycerol content and insulin-stimulated glucose uptake in comparison with dietary saturated fatty acids. Male weanling WNIN rats were divided into three groups and fed for 3 months on one of the following diets containing 10 % oil differing in fatty acid composition: control diet, saturated fatty acid diet and trans fatty acid diet. Dietary trans fatty acids increased the intramyocellular triacylglycerols and decreased the ratio of 20 : 4n-6 to 18 : 2n-6 and long-chain PUFA levels (20 %) in diaphragm phospholipids, indicating inhibition of PUFA biosynthesis. However, saturated fatty acids decreased both 18 : 2n-6 and 20 : 4n-6 without change in the ratio. Trans fatty acid-induced alterations in diaphragm phospholipid fatty acid composition and intramyocellular triacylglycerol content were associated with decreased insulin-stimulated glucose transport in the diaphragm. These observations suggest that dietary trans fatty acids decrease diaphragm insulin sensitivity, possibly due to increased intramyocellular triacylglycerol accumulation and decreased long-chain PUFA in phospholipids.

MeSH terms

  • Animals
  • Diaphragm / metabolism*
  • Diet
  • Dietary Fats / administration & dosage
  • Dietary Fats / pharmacology*
  • Fatty Acids / metabolism*
  • Fatty Acids, Unsaturated / metabolism
  • Glucose / metabolism*
  • Male
  • Phospholipids / metabolism*
  • Rats
  • Trans Fatty Acids / administration & dosage
  • Trans Fatty Acids / pharmacology*
  • Triglycerides / metabolism*


  • Dietary Fats
  • Fatty Acids
  • Fatty Acids, Unsaturated
  • Phospholipids
  • Trans Fatty Acids
  • Triglycerides
  • Glucose