Prime-boost vaccination with plasmid DNA and a chimeric adenovirus type 5 vector with type 35 fiber induces protective immunity against HIV

Gene Ther. 2005 Dec;12(24):1769-77. doi: 10.1038/


Immunization involving a DNA vaccine prime followed by an adenovirus type 5 (Ad5) boost elicited a protective immune response against SHIV challenge in monkeys. However, the hepatocellular tropism of Ad5 limits the safety of this viral vector. This study examines the safety and immunogenicity of a replication-defective chimeric Ad5 vector with the Ad35 fiber (Ad5/35) in BALB/c mice and rhesus monkeys. This novel Ad5/35 vector showed minimal hepatotoxicity after intramuscular administration with the novel Ad5/35 vector. In addition, an Ad5/35 vector expressing HIV Env gp160 protein (Ad5/35-HIV) generated strong HIV-specific immune responses in both animal models. Priming with a DNA vaccine followed by Ad5/35-HIV boosting yielded protection against a gp160-expressing vaccinia virus challenge in BALB/c mice. The Ad5/35-HIV vector was significantly less susceptible to the pre-existing Ad5 immunity than a comparable Ad5 vector. These findings indicate that an Ad5/35 vector-based HIV vaccine may be of considerable value for clinical use.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • AIDS Vaccines / administration & dosage*
  • Adenoviridae / genetics
  • Animals
  • Antibodies, Viral / blood
  • DNA, Viral / administration & dosage
  • Female
  • Genetic Therapy / methods*
  • Genetic Vectors / administration & dosage
  • Genetic Vectors / genetics
  • HIV Infections / prevention & control*
  • HIV-1* / genetics
  • HIV-1* / immunology
  • Immunization / methods*
  • Immunization, Secondary
  • Macaca mulatta
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Models, Animal
  • Neutralization Tests
  • Vaccines, DNA / administration & dosage*
  • Vaccinia virus / physiology
  • Viral Proteins / genetics
  • Virus Physiological Phenomena


  • AIDS Vaccines
  • Antibodies, Viral
  • DNA, Viral
  • Vaccines, DNA
  • Viral Proteins