Adrenomedullin modulates COX-2 and HGF expression in reserpine-injuried gastric mucosa in the rat

Eur J Pharmacol. 2005 Aug 22;518(2-3):221-6. doi: 10.1016/j.ejphar.2005.06.001.

Abstract

Here we show the increased hepatocyte growth factor (HGF) and cyclooxygenase-2 (COX-2) expression in gastric mucosa of rats which have developed a reserpine-induced ulcer. Such an increase of HGF and COX-2 expression was blunted in rats pretreated with adrenomedullin. Pretreatment with adrenomedullin and the adrenomedullin22-52 fragment did not result in changes of HGF and COX-2 expression, compared to the reserpine and adrenomedullin treated group. Pretreatment with adrenomedullin and the calcitonin gene-related peptide8-37 fragment (CGRP8-37) increased HGF and COX-2 expression, compared to the reserpine and adrenomedullin treated group. Our results suggest that the inhibitory effect of adrenomedullin on the expression of HGF and COX-2 is mediated by CGRP receptors.

Publication types

  • Comparative Study

MeSH terms

  • Adrenomedullin
  • Analysis of Variance
  • Animals
  • Blotting, Western
  • Calcitonin Gene-Related Peptide / pharmacology
  • Cyclooxygenase 2
  • Gastric Mucosa / drug effects*
  • Gastric Mucosa / metabolism
  • Gastric Mucosa / pathology
  • Hepatocyte Growth Factor / biosynthesis*
  • Immunohistochemistry
  • Male
  • Peptide Fragments / pharmacology
  • Peptides / pharmacology*
  • Prostaglandin-Endoperoxide Synthases / biosynthesis*
  • Rats
  • Rats, Sprague-Dawley
  • Reserpine / toxicity*

Substances

  • Peptide Fragments
  • Peptides
  • calcitonin gene-related peptide (8-37)
  • Adrenomedullin
  • Hepatocyte Growth Factor
  • Reserpine
  • Cyclooxygenase 2
  • Prostaglandin-Endoperoxide Synthases
  • Calcitonin Gene-Related Peptide