Ran-GTP regulates kinetochore attachment in somatic cells

Cell Cycle. 2005 Sep;4(9):1161-5. doi: 10.4161/cc.4.9.1979. Epub 2005 Sep 28.

Abstract

The Ran GTPase controls multiple mitotic processes in Xenopus egg extracts, including mitotic checkpoints, spindle assembly and post-mitotic nuclear envelope reassembly. We have analyzed Ran's role in somatic cells. We uncovered a novel mitotic role of Ran-GTP, involving the Crm1 nuclear export receptor. This pathway is an important mode of Ran-GTP function during mitosis in mammalian somatic cells, which mediates the recruitment of the RanGAP1/RanBP2 complex to kinetochores and maintains the microtubule-based fibers connecting kinetochores to spindle poles (k-fibers). Here we discuss potential implications of these findings for normal k-fiber assembly.

Publication types

  • Review

MeSH terms

  • Active Transport, Cell Nucleus
  • Animals
  • Cell Cycle Proteins / metabolism
  • GTPase-Activating Proteins / metabolism
  • Genome
  • Guanine Nucleotide Exchange Factors / metabolism
  • Guanosine Triphosphate / chemistry
  • Karyopherins / metabolism
  • Kinetochores / metabolism*
  • Microtubules / metabolism
  • Mitosis
  • Models, Biological
  • Molecular Chaperones / metabolism
  • Nuclear Pore Complex Proteins / metabolism
  • Nuclear Proteins / metabolism
  • Protein Binding
  • Receptors, Cytoplasmic and Nuclear / metabolism
  • Spindle Apparatus / metabolism
  • Xenopus
  • Xenopus Proteins / metabolism
  • ran GTP-Binding Protein / physiology*

Substances

  • Cell Cycle Proteins
  • GTPase-Activating Proteins
  • Guanine Nucleotide Exchange Factors
  • Karyopherins
  • Molecular Chaperones
  • Nuclear Pore Complex Proteins
  • Nuclear Proteins
  • RCC1 protein, Xenopus
  • RanGAP1 protein, Xenopus
  • Receptors, Cytoplasmic and Nuclear
  • Xenopus Proteins
  • exportin 1 protein
  • ran-binding protein 2
  • Guanosine Triphosphate
  • ran GTP-Binding Protein