The effect of tunicamycin on the protease activity of GP63 from Leishmania major

C Hernandez; S Manenti; A G Hernandez

doi:10.1007/BF00419752

The effect of tunicamycin on the protease activity of GP63 from Leishmania major

Mol Biol Rep. 1992 May;16(2):81-4. doi: 10.1007/BF00419752.

Authors

C Hernandez¹, S Manenti, A G Hernandez

Affiliation

¹ Departamento de Biologia Celular, Facultad de Ciencias, Universidad Central de Venezuela, Caracas.

PMID: 1608400
DOI: 10.1007/BF00419752

Abstract

The protease activity of gp63 from L. major was studied in relation to tunicamycin induced N-deglycosylation. It was found that after tunicamycin treatment, a N-deglycosylated product of gp63 with protease activity is present at the cell surface of Leishmania promastigote.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amidohydrolases
Animals
Electrophoresis, Polyacrylamide Gel
Leishmania tropica / enzymology*
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
Metalloendopeptidases / drug effects*
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Solubility
Tunicamycin / pharmacology*

Substances

Tunicamycin
Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
Metalloendopeptidases
glycoprotein gp63, Leishmania
Amidohydrolases
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase