ProRule: a new database containing functional and structural information on PROSITE profiles

Bioinformatics. 2005 Nov 1;21(21):4060-6. doi: 10.1093/bioinformatics/bti614. Epub 2005 Aug 9.


Motivation: Increase the discriminatory power of PROSITE profiles to facilitate function determination and provide biologically relevant information about domains detected by profiles for the annotation of proteins.

Summary: We have created a new database, ProRule, which contains additional information about PROSITE profiles. ProRule contains notably the position of structurally and/or functionally critical amino acids, as well as the condition they must fulfill to play their biological role. These supplementary data should help function determination and annotation of the UniProt Swiss-Prot knowledgebase. ProRule also contains information about the domain detected by the profile in the Swiss-Prot line format. Hence, ProRule can be used to make Swiss-Prot annotation more homogeneous and consistent. The format of ProRule can be extended to provide information about combination of domains.

Availability: ProRule can be accessed through ScanProsite at A file containing the rules will be made available under the PROSITE copyright conditions on our ftp site ( by the next PROSITE release.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Database Management Systems*
  • Databases, Protein*
  • Information Storage and Retrieval / methods*
  • Molecular Sequence Data
  • Protein Conformation
  • Proteins / chemistry*
  • Proteins / classification
  • Proteins / metabolism*
  • Sequence Analysis, Protein / methods*
  • Structure-Activity Relationship


  • Proteins