Attenuation of acid induced oesophagitis in VR-1 deficient mice

Gut. 2006 Jan;55(1):34-40. doi: 10.1136/gut.2005.066795. Epub 2005 Aug 9.


Background and aims: Activation of the vanilloid receptor subtype 1 (VR-1) results in release of proinflammatory peptides which initiate an inflammatory cascade known as neurogenic inflammation. We investigated its role in an acute model of surgically induced oesophagitis.

Methods: Oesophagitis was induced by pyloric ligation in wild-type and VR-1 deficient mice. A subset of animals were administered the VR-1 antagonist capsazepine, famotidine, or omeprazole one hour before surgery. Five hours after surgery, myeloperoxidase activity (MPO), histological damage scores, intragastric pH, and immunocytochemical analysis of substance P (SP) receptor endocytosis were determined.

Results: Oesophagitis induced knockout mice exhibited significantly lower levels of MPO activity, histological damage scores, and SP receptor endocytosis than wild-type mice. Inflammatory parameters were significantly reduced by acid inhibition and capsazepine in wild-type mice.

Conclusions: We conclude that acute acid induced oesophagitis is reduced in animals lacking VR-1. This suggests that acid induced oesophagitis may act through VR-1 and that inhibition of the receptor may reduce inflammation.

MeSH terms

  • Acute Disease
  • Animals
  • Anti-Ulcer Agents / therapeutic use
  • Capsaicin / analogs & derivatives
  • Capsaicin / therapeutic use
  • Endocytosis
  • Esophagitis / pathology
  • Esophagitis / physiopathology
  • Esophagitis / prevention & control*
  • Famotidine / therapeutic use
  • Hydrogen-Ion Concentration
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Microscopy, Confocal
  • Omeprazole / therapeutic use
  • Peroxidase / metabolism
  • Receptors, Neurokinin-1 / metabolism
  • Severity of Illness Index
  • TRPV Cation Channels / antagonists & inhibitors
  • TRPV Cation Channels / deficiency
  • TRPV Cation Channels / genetics
  • TRPV Cation Channels / physiology*


  • Anti-Ulcer Agents
  • Receptors, Neurokinin-1
  • TRPV Cation Channels
  • TRPV1 receptor
  • Famotidine
  • Peroxidase
  • Omeprazole
  • capsazepine
  • Capsaicin