Production and characterization of two monoclonal antibodies directed against the integrin beta 1 chain

Tumori. 1992 Feb 29;78(1):1-4. doi: 10.1177/030089169207800101.

Abstract

The production and characterization of two new monoclonal antibodies (MAbs), designated MAR4 and MAR5, raised against the partially purified alpha 5 beta 1 integrin, are described. The reactivity of these 2 MAbs on tumor cell lines indicated that they reacted on all the cells expressing the beta 1 subunit independently of the alpha 5 expression. Both MAbs were found to immunoprecipitate on 3 cell lines, a protein of 120 KD corresponding to the molecular weight be the beta 1 chain, in addition to proteins of other MW corresponding to the alpha subunits differentially expressed by these cells. The cross-competition experiments showed that MAR4 and MAR5 recognize the same epitope. These 2 MAbs seem to be useful reagents for the characterization of the VLA expression in tumor cells.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / analysis
  • Antibodies, Monoclonal / biosynthesis*
  • Antibodies, Monoclonal / isolation & purification
  • Binding, Competitive
  • Cell Line
  • Fluorescent Antibody Technique
  • Humans
  • Hybridomas / immunology
  • Integrins / immunology*
  • Iodine Radioisotopes
  • Mice
  • Mice, Inbred BALB C
  • Precipitin Tests
  • Tumor Cells, Cultured / immunology

Substances

  • Antibodies, Monoclonal
  • Integrins
  • Iodine Radioisotopes