Expression of interferon inducible protein-10 in pancreas of mice

World J Gastroenterol. 2005 Aug 14;11(30):4750-2. doi: 10.3748/wjg.v11.i30.4750.

Abstract

Aim: To investigate the expression of interferon inducible protein-10 (IP-10) in pancreas of mice and to discuss its possible role in the pathogenesis of type 1 diabetes.

Methods: Non-obese diabetic (NOD) mice were used as experiment group and BALB/c mice as non-diabetic prone model. Immunohistochemistry method was used to evaluate the expression of IP-10 in the pancreas of NOD mice and BALB/c mice. Immunoelectron microscope was used to show the location of IP-10 in pancreatic islet beta cells.

Results: Pancreatic islets were positively stained in all the NOD mice. Insulitis could be found in mice at the age of 4 wk. The weakly positive results were found in control group with no insulitis. Immunoelectron microscopy further demonstrated that IP-10 was produced by pancreatic beta cells and stored in cytoplasm of the cells.

Conclusion: IP-10 can be largely produced in pancreatic islets of NOD mice at the age of 2 wk when there is no significant insulitis, and may play an important part in the pathogenesis of type 1 diabetes by attracting immune cells to infiltrate the pancreatic islets.

MeSH terms

  • Animals
  • Chemokine CXCL10
  • Chemokines, CXC / metabolism*
  • Diabetes Mellitus, Type 1 / etiology
  • Diabetes Mellitus, Type 1 / immunology
  • Female
  • Interferon-gamma / metabolism
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred NOD
  • Microscopy, Immunoelectron
  • Pancreas / immunology*
  • Pancreas / ultrastructure

Substances

  • Chemokine CXCL10
  • Chemokines, CXC
  • Interferon-gamma