Analysis of adenocarcinoma of the colon and rectum: detection of human papillomavirus (HPV) DNA by polymerase chain reaction

Colorectal Dis. 2005 Sep;7(5):492-5. doi: 10.1111/j.1463-1318.2005.00774.x.


Objective: The aim of the present work was to evaluate the presence of human papillomavirus genotypes in malignant and normal mucosa of the colon and rectum in order to determine if a relationship exists between HPV infection and colon neoplasms.

Materials and methods: Thirty normal colon tissues and 54 sporadic adenocarcinomas were screened for HPV positivity using nested-PCR. Detection of viral types 6, 11, 16, 18, 33, 34 and 51 was performed by the LIS-SSCP (Low Ionic Strength-Single Strand Conformational Polymorphism) procedure.

Results: Significant differences in high risk HPV infection were found between normal samples and adenocarcinomas (P < 0.001). Among the cases, an inverse association between HPV infection and Dukes staging was also found (P = 0.020). Finally, there was no significant association between HPV and some classical clinicopathological features, although a gradient of infection form rectum to cecum was evident.

Conclusion: The present study demonstrates that HPV may infect the glandular mucosa of the colon and suggests a possible association between HPV and colorectal cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / genetics
  • Adenocarcinoma / virology*
  • Aged
  • Aged, 80 and over
  • Chi-Square Distribution
  • Colonic Neoplasms / genetics
  • Colonic Neoplasms / virology*
  • DNA, Viral / analysis
  • Female
  • Genotype
  • Humans
  • Male
  • Middle Aged
  • Papillomaviridae / genetics
  • Papillomaviridae / isolation & purification*
  • Papillomavirus Infections / diagnosis*
  • Papillomavirus Infections / genetics
  • Polymerase Chain Reaction
  • Polymorphism, Single-Stranded Conformational
  • Rectal Neoplasms / genetics
  • Rectal Neoplasms / virology*


  • DNA, Viral