Free uptake of cell-penetrating peptides by fission yeast

FEBS Lett. 2005 Aug 29;579(21):4873-8. doi: 10.1016/j.febslet.2005.07.064.


An increasing number of peptides translocate the plasma membrane of mammalian cells promising new avenues for drug delivery. However, only a few examples are known to penetrate the fungal cell wall. We compared the capacity of different fluorophore-labelled peptides to translocate into fission yeast and human cells and determined their intracellular distribution. Most of the 20 peptides tested were able to enter human cells, but only one, transportan 10 (TP10), efficiently penetrated fission yeast and was distributed uniformly inside the cells. The results show that the fungal cell wall may reduce, but does not block peptide uptake.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Wall / metabolism
  • Drug Carriers
  • Endocytosis / physiology*
  • Fluorescent Dyes / metabolism
  • Galanin
  • HeLa Cells
  • Humans
  • Peptides / metabolism*
  • Recombinant Fusion Proteins / metabolism*
  • Schizosaccharomyces / cytology
  • Schizosaccharomyces / metabolism*
  • Wasp Venoms


  • Drug Carriers
  • Fluorescent Dyes
  • Peptides
  • Recombinant Fusion Proteins
  • Wasp Venoms
  • transportan
  • Galanin