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. 2006 Jan 11;78(7):724-9.
doi: 10.1016/j.lfs.2005.05.064. Epub 2005 Aug 18.

Knockdown of Ki-67 by Small Interfering RNA Leads to Inhibition of Proliferation and Induction of Apoptosis in Human Renal Carcinoma Cells

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Knockdown of Ki-67 by Small Interfering RNA Leads to Inhibition of Proliferation and Induction of Apoptosis in Human Renal Carcinoma Cells

Jun-Nian Zheng et al. Life Sci. .

Abstract

To investigate the effect of small-interfering RNA (siRNA) targeted against Ki-67, which is an attractive molecular target for cancer therapy, on inhibiting Ki-67 expression and cell proliferation in human renal carcinoma cells (HRCCs), siRNAs were used to inhibit the expression of Ki-67 in HRCCs. Ki-67 mRNA levels were detected by RT-PCR and in situ hybridization analysis. Ki-67 protein levels were detected by Western blot and immunocytochemistry analysis. TUNEL assay was used to measure the apoptosis of carcinoma cells. Results of RT-PCR and in situ hybridization demonstrated reduction of Ki-67 mRNA expression in Ki-67 siRNAs treated 786-0 cells. Similar reduction in Ki-67 protein measured by Western blot and immunocytochemistry was observed in cells transfected with Ki-67 siRNA. Ki-67-siRNA treatment of HRCCs resulted in specific inhibition of proliferation and increased apoptotic cell death. From these findings we conclude that inhibition of Ki-67 expression by siRNA may be a reasonable approach in renal cancer therapy.

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