Methylation protects miRNAs and siRNAs from a 3'-end uridylation activity in Arabidopsis

Curr Biol. 2005 Aug 23;15(16):1501-7. doi: 10.1016/j.cub.2005.07.029.

Abstract

Small RNAs of 21-25 nucleotides (nt), including small interfering RNAs (siRNAs) and microRNAs (miRNAs), act as guide RNAs to silence target-gene expression in a sequence-specific manner. In addition to a Dicer homolog, DCL1, the biogenesis of miRNAs in Arabidopsis requires another protein, HEN1. miRNAs are reduced in abundance and increased in size in hen1 mutants. We found that HEN1 is a miRNA methyltransferase that adds a methyl group to the 3'-most nucleotide of miRNAs, but the role of miRNA methylation was unknown. Here, we show that siRNAs from sense transgenes, hairpin transgenes, and transposons or repeat sequences, as well as a new class of siRNAs known as trans-acting siRNAs, are also methylated in vivo by HEN1. In addition, we show that the size increase of small RNAs in the hen1-1 mutant is due to the addition of one to five U residues to the 3' ends of the small RNAs. Therefore, a novel uridylation activity targets the 3' ends of unmethylated miRNAs and siRNAs in hen1 mutants. We conclude that 3'-end methylation is a common step in miRNA and siRNA metabolism and likely protects the 3' ends of the small RNAs from the uridylation activity.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics*
  • Arabidopsis Proteins / metabolism*
  • Cell Cycle Proteins / metabolism
  • Cloning, Molecular
  • Methylation
  • MicroRNAs / metabolism*
  • Molecular Sequence Data
  • RNA 3' End Processing / physiology*
  • RNA, Small Interfering / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Ribonuclease III / metabolism
  • Uridine / metabolism

Substances

  • Arabidopsis Proteins
  • Cell Cycle Proteins
  • HEN1 protein, Arabidopsis
  • MicroRNAs
  • RNA, Small Interfering
  • DCL1 protein, Arabidopsis
  • Ribonuclease III
  • Uridine