Statins inhibit hypoxia-induced endothelial proliferation by preventing calcium-induced ROS formation

Atherosclerosis. 2006 Apr;185(2):290-6. doi: 10.1016/j.atherosclerosis.2005.06.035. Epub 2005 Aug 19.

Abstract

Pathological hypoxia plays an important role in many diseases, such as atherosclerosis, cancer, and rheumatoid arthritis. The aim of the present study was to examine the effects of different statins on hypoxia-induced endothelial cell signalling. Human umbilical cord vein endothelial cells (HUVEC) were treated with NaCN (CN, 2.5 mmol/l) to simulate a transient hypoxia. The CN-induced increase of endothelial cell numbers was significantly (n = 10, p < 0.01) reduced by the Ca(2+) chelator BAPTA (10 micromol/l), or the reactive oxygen species (ROS) scavenger N-acetylcysteine (ACC, 1 mmol/l), or the NAD(P)H-oxidase inhibitor diphenyleneiodonium (DPI, 5 micromol/l). In detail, cell numbers were (in percentage of control): 163.24 (CN), 90.06 (CN+ACC), 92.06 (CN+DPI). Intracellular-Ca(2+) and -ROS, analysed by fluorescence imaging, were significantly increased by CN. Interestingly, the CN-induced increase of ROS was in part Ca(2+)-dependent, whereas the Ca(2+) increase was not ROS-dependent. Simvastatin (5 micromol/l), fluvastatin (2.5 micromol/l), and cerivastatin (0.1 micromol/l) all reduced CN-induced proliferation, ROS generation and Ca(2+) increase. Cell viability was not reduced by the statins and the antiproliferative effect was completely reversed by mevalonate (500 micromol/l). In conclusion our study demonstrates that statins block hypoxia-associated endothelial proliferation by preventing the increase of Ca(2+) and ROS.

MeSH terms

  • Acetylcysteine / pharmacology
  • Calcium / metabolism*
  • Cell Hypoxia / drug effects
  • Cell Proliferation / drug effects*
  • Cell Survival
  • Cells, Cultured
  • Chelating Agents / pharmacology
  • Egtazic Acid / analogs & derivatives
  • Egtazic Acid / pharmacology
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / drug effects*
  • Endothelium, Vascular / metabolism
  • Free Radical Scavengers / pharmacology
  • Humans
  • Hydroxymethylglutaryl-CoA Reductase Inhibitors / pharmacology*
  • Nitric Oxide Synthase / antagonists & inhibitors
  • Onium Compounds / pharmacology
  • Reactive Oxygen Species / metabolism*
  • Sodium Cyanide / pharmacology

Substances

  • Chelating Agents
  • Free Radical Scavengers
  • Hydroxymethylglutaryl-CoA Reductase Inhibitors
  • Onium Compounds
  • Reactive Oxygen Species
  • Egtazic Acid
  • diphenyleneiodonium
  • Nitric Oxide Synthase
  • 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid
  • Sodium Cyanide
  • Calcium
  • Acetylcysteine