Group V allergens in grass pollens: IV. Similarities in amino acid compositions and NH2-terminal sequences of the group V allergens from Lolium perenne, Poa pratensis and Dactylis glomerata

Clin Exp Allergy. 1992 Apr;22(4):491-7. doi: 10.1111/j.1365-2222.1992.tb00152.x.


Monoclonal antibodies (PpV4) raised against Phleum pratense group V allergen were used for immuno-affinity chromatography of cross-reacting group V allergens from related grass species. Fractions enriched in group V allergen were obtained from Lolium perenne, Poa pratense and Dactylis glomerata extracts. The major components in these fractions were found in the Mwr range 25-28 kD. IgE binding to these components was shown using a pool of grass allergic sera, by SDS-PAGE immunoblotting. These fractions were electroblotted from tricine SDS-PAGE gels onto a polyvinylidene-difluoride membrane and selected group V bands were directly cut out and used for amino acid analysis and NH2-terminal sequencing. Both the amino acid compositions and the NH2-terminal sequences obtained for each group V allergen were almost similar to each other and to the sequence and composition of the previously described allergen Phl p V from Phleum pratense. A common trait of the investigated allergens, is the very high contents of alanine (25-32%) and the presence of the modified amino acid, hydroxyproline.

Publication types

  • Comparative Study

MeSH terms

  • Allergens / chemistry*
  • Allergens / classification
  • Amino Acid Sequence
  • Amino Acids / analysis
  • Animals
  • Antibodies, Monoclonal
  • Cross Reactions
  • Humans
  • Immunochemistry
  • Mice
  • Molecular Sequence Data
  • Poaceae
  • Pollen / chemistry*
  • Rhinitis, Allergic, Seasonal / immunology
  • Sequence Homology, Nucleic Acid


  • Allergens
  • Amino Acids
  • Antibodies, Monoclonal