Isolation of yeast mitochondria

Methods Mol Biol. 2006;313:33-9. doi: 10.1385/1-59259-958-3:033.


Often preparations of isolated organelles contain other, unwanted, cellular components. For biochemical experiments to determine the localization of newly identified proteins, or to determine the whole set of proteins (or the proteome) from a desired organelle, these unwanted components often confuse the resulting data. For these types of studies, it is crucial to have highly pure fractions of the desired organelle. Here we describe a protocol for purification of mitochondria from Saccharomyces cerevisiae cells devoid of contamination from other cellular compartments.

MeSH terms

  • Blotting, Western
  • Cell Fractionation / methods*
  • Indicators and Reagents
  • Mitochondria / chemistry*
  • Mycology / methods
  • Saccharomyces cerevisiae / chemistry*
  • Saccharomyces cerevisiae / growth & development
  • Saccharomyces cerevisiae Proteins / isolation & purification


  • Indicators and Reagents
  • Saccharomyces cerevisiae Proteins