Yeast transformation by the LiAc/SS Carrier DNA/PEG method

Methods Mol Biol. 2006;313:107-20. doi: 10.1385/1-59259-958-3:107.

Abstract

The technique for the transformation of Saccharomyces cerevisiae using the LiAc/SS Carrier DNA/PEG method is described. We describe a rapid method, for use when large numbers of transformants are not necessary. A high-efficiency method for the generation of large numbers of transformants is also given. A method for the transformation of plasmid libraries, which includes yeast two-hybrid applications, also is listed to aid the reader in generating transformants to effectively cover the library complexity. Finally, a protocol for transformation using a 96-well format is included for transformation applications that require it.

MeSH terms

  • Acetates
  • DNA, Recombinant / genetics
  • DNA, Single-Stranded / genetics
  • Gene Library
  • Mycology / methods
  • Plasmids / genetics
  • Polyethylene Glycols
  • Saccharomyces cerevisiae / genetics*
  • Transformation, Genetic*
  • Two-Hybrid System Techniques

Substances

  • Acetates
  • DNA, Recombinant
  • DNA, Single-Stranded
  • Polyethylene Glycols
  • lithium acetate