A series of events occur in plant following herbivore and pathogen attack, which includes changes in ion fluxes changing through plasma membrane, rapid increasing in cytoplasmic Ca(2+) concentration, production of active oxygen species, and protein phosphorylation. These early signaling events may trigger a number of responses through salicylate, jasmonate, and ethylene signaling pathways, activating the defense responsive genes, such as PR1, BFL2. The products of these defensive genes could act on the herbivore insects. Plant defense reactions are regulated by complex networks of inter- and intra-cellular signaling pathways. They could be antagonistic or synergic and provide effective defense for plant against various biotic stresses.