Abstract
The contribution of plasma membrane-associated glucocorticoid receptor (GR) to transcriptional signaling is unclear. We observed GR in low-density detergent-resistant membrane (DRM) rafts derived from human hepatoma Hep3B cells in complexes with caveolin-1, HSP90, and STAT3. In transient transfection assays, GR-stimulated transcriptional signaling was reversibly inhibited by membrane-raft disrupters filipin III and progesterone. These data provide clear evidence for a functional contribution of DRM-associated GR to transcriptional signaling.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Caveolin 1
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Caveolins / metabolism
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Cell Line, Tumor
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Cell Membrane / metabolism
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Filipin / pharmacology
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Humans
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Octoxynol / chemistry
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Oligonucleotides
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Receptors, Glucocorticoid / antagonists & inhibitors
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Receptors, Glucocorticoid / metabolism*
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Signal Transduction*
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Transcription, Genetic*
Substances
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CAV1 protein, human
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Caveolin 1
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Caveolins
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Oligonucleotides
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Receptors, Glucocorticoid
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Filipin
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Octoxynol